Anti-inflammatory activity of novel natural plant extracts composition—Levidor^TM

Abstract Introduction Due to the severe side effects of non-steroidal anti-inflammatory drugs, there is an urgent need find new natural or synthetic chemicals that could act as selective inhibitors cyclooxygenase-2 (COX-2). The most valuable source bioactive compounds and potential drugs are plants. present study aimed at investigation Levidor TM , a patented composition based on oil from seeds Nigella sativa extract root Angelica archangelica, regarding its molecular level. Materials Methods Studies were carried out with use murine macrophages (RAW 264.7), human monocytes (U937), rat pheochromocytoma (PC-12) cell lines. cytotoxicity was evaluated by MTT assay. concentration pro-inflammatory cytokines (IL-6, TNF- α ) ELISA. Moreover, analysis COX-1 COX-2 mRNA expression levels RT-PCR, well protein level Western blot immunofluorescent staining, assessed. Results did not impact growth showed effect in study. It able decrease IL-6, suppress transcription COX-2, whereas it had no COX-1. Conclusions Obtained results exhibits strong properties may provide significant alternative traditionally used medicines acute pain chronic inflammation.